Abstract
In order to differentiate T cells in vitro, co-culture systems with Notch ligand-expressing feeder cells have been in use for a long time. Here we describe a feeder-free culture condition for differentiating T cells from hematopoietic cells that are cultured on Fc-DLL4-coated plate with T-lineage cytokines. This condition is capable of efficiently differentiating hematopoietic progenitor cells (HPCs) to immature T cells expressing both CD4 and CD8. To mature those cells into functional T cells, further stimulation and culture is necessary.
Keywords:
Feeder-free differentiation; Immunotherapy; Regenerative medicine; T cell; iPS cell.
MeSH terms
-
Adaptor Proteins, Signal Transducing / genetics
-
Adaptor Proteins, Signal Transducing / metabolism
-
Calcium-Binding Proteins / genetics
-
Calcium-Binding Proteins / metabolism
-
Cell Culture Techniques / instrumentation
-
Cell Culture Techniques / methods*
-
Cell Differentiation*
-
Cells, Cultured / physiology
-
Culture Media / metabolism*
-
Cytokines / metabolism
-
Humans
-
Immunoglobulin Fc Fragments / genetics
-
Immunoglobulin Fc Fragments / metabolism
-
Induced Pluripotent Stem Cells / physiology
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
T-Lymphocytes / physiology
Substances
-
Adaptor Proteins, Signal Transducing
-
Calcium-Binding Proteins
-
Culture Media
-
Cytokines
-
DLL4 protein, human
-
Immunoglobulin Fc Fragments
-
Recombinant Fusion Proteins