Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database

Charlotte A Huber; Valentin Pflüger; Sarah Reed; Kyra Cottrell; Hanna E Sidjabat; Ama Ranasinghe; Hosam M Zowawi; Patrick Harris; David L Paterson

doi:10.1016/j.mimet.2019.105685

Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database

J Microbiol Methods. 2019 Sep:164:105685. doi: 10.1016/j.mimet.2019.105685. Epub 2019 Aug 7.

Authors

Charlotte A Huber¹, Valentin Pflüger², Sarah Reed³, Kyra Cottrell³, Hanna E Sidjabat³, Ama Ranasinghe³, Hosam M Zowawi⁴, Patrick Harris³, David L Paterson³

Affiliations

¹ The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia. Electronic address: [email protected].
² Mabritec SA, Riehen, Switzerland.
³ The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia.
⁴ The University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia; World Health Organization Collaborating Centre for Infection Prevention and Control, Riyadh, Saudi Arabia.

PMID: 31400360
DOI: 10.1016/j.mimet.2019.105685

Abstract

In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification.

MeSH terms

Bacteria / genetics
Bacteria / isolation & purification*
Bacterial Proteins / genetics
Bacterial Typing Techniques / instrumentation
Bacterial Typing Techniques / methods*
DNA, Bacterial / genetics
Databases, Factual
RNA, Ribosomal, 16S / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Bacterial Proteins
DNA, Bacterial
RNA, Ribosomal, 16S