Pre-exposure of human B cells to recombinant IL-1 enhances subsequent proliferation

J Immunol. 1988 Nov 15;141(10):3398-404.

Abstract

The reported effects of the monocyte-derived cytokine IL-1 on human B lymphocytes are both varied and controversial. IL-1 has been reported to augment both proliferation and Ig secretion of previously activated human B cells. In the present study highly purified splenic B cells were cultured with rIL-1 before, simultaneously with, and after the addition of the polyclonal B cell mitogen, anti-Ig. rIL-1 had no significant effect on B cell proliferation when added simultaneously with or after B cell activation with anti-Ig. However, incubation of splenic B cells with rIL-1 for 24 h before stimulation with anti-Ig appeared to enhance mitogenesis. With the observation that rIL-1 exerted effects on resting B cells, the effect of rIL-1 on several events which accompany B cell activation was examined. rIL-1 failed to stimulate RNA synthesis, effect increases in cell size or intracellular Ca2+ levels, or lead to the hyperexpression of MHC class II or B cell activation Ag. These studies suggest that rIL-1 does not activate B cells but primes them to respond to subsequent activation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Anti-Idiotypic
  • B-Lymphocytes / drug effects*
  • B-Lymphocytes / immunology
  • Cells, Cultured
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-1 / pharmacology*
  • Interleukin-2
  • Interleukin-4
  • Interleukins
  • Lymphocyte Activation / drug effects*
  • Palatine Tonsil / drug effects
  • Palatine Tonsil / immunology
  • Recombinant Proteins / pharmacology*
  • Spleen / drug effects
  • Spleen / immunology
  • Time Factors

Substances

  • Antibodies, Anti-Idiotypic
  • Interleukin-1
  • Interleukin-2
  • Interleukins
  • Recombinant Proteins
  • Interleukin-4
  • Interferon-gamma