Implementation of HPV-based Cervical Cancer Screening Combined with Self-sampling Using a Midwifery Network Across Rural Greece: The GRECOSELF Study

Cancer Prev Res (Phila). 2019 Oct;12(10):701-710. doi: 10.1158/1940-6207.CAPR-19-0192. Epub 2019 Aug 19.

Abstract

Self-sampling for human papillomavirus (HPV) testing is an alternative to physician sampling particularly for cervical cancer screening nonattenders. The GRECOSELF study is a nationwide observational cross-sectional study aiming to suggest a way to implement HPV-DNA testing in conjunction with self-sampling for cervical cancer screening in Greece, utilizing a midwifery network. Women residing in remote areas of Greece were approached by midwives, of a nationwide network, and were provided with a self-collection kit (dry swab) for cervicovaginal sampling and asked to answer a questionnaire about their cervical cancer screening history. Each sample was tested for high-risk (hr) HPV with the Cobas HPV test. HrHPV-Positive women were referred to undergo colposcopy and, if needed, treatment according to colposcopy/biopsy results. Between May 2016 and November 2018, 13,111 women were recruited. Of these, 12,787 women gave valid answers in the study questionnaire and had valid HPV-DNA results; hrHPV prevalence was 8.3%; high-grade cervical/vaginal disease or cancer prevalence was 0.6%. HrHPV positivity rate decreased with age from 20.7% for women aged 25-29 years to 5.1% for women aged 50-60 years. Positive predictive value for hrHPV testing and for HPV16/18 genotyping ranged from 5.0% to 11.6% and from 11.8% to 27.0%, respectively, in different age groups. Compliance to colposcopy referral rate ranged from 68.6% (for women 25-29) to 76.3% (for women 40-49). For women residing in remote areas of Greece, the detection of hrHPV DNA with the Cobas HPV test, on self-collected cervicovaginal samples using dry cotton swabs, which are provided by visiting midwives, is a promising method for cervical cancer secondary prevention.

Publication types

  • Observational Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Colposcopy / statistics & numerical data
  • Community Networks / organization & administration
  • Community Networks / standards
  • Cross-Sectional Studies
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Diagnostic Self Evaluation
  • Early Detection of Cancer / methods
  • Early Detection of Cancer / standards
  • Early Detection of Cancer / statistics & numerical data
  • Female
  • Greece / epidemiology
  • Human Papillomavirus DNA Tests* / methods
  • Human Papillomavirus DNA Tests* / standards
  • Human Papillomavirus DNA Tests* / statistics & numerical data
  • Humans
  • Implementation Science
  • Mass Screening / methods
  • Mass Screening / organization & administration*
  • Mass Screening / standards
  • Middle Aged
  • Midwifery / methods
  • Midwifery / organization & administration*
  • Nurse Midwives / organization & administration
  • Nurse Midwives / standards
  • Nurse Midwives / statistics & numerical data
  • Nurse's Role
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / pathology
  • Rural Population / statistics & numerical data
  • Specimen Handling / methods*
  • Specimen Handling / standards
  • Specimen Handling / statistics & numerical data
  • Uterine Cervical Dysplasia / diagnosis*
  • Uterine Cervical Dysplasia / epidemiology
  • Uterine Cervical Dysplasia / etiology
  • Uterine Cervical Neoplasms / diagnosis*
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / etiology
  • Vaginal Smears / methods
  • Vaginal Smears / statistics & numerical data
  • Young Adult

Substances

  • DNA, Viral