Validation of a formula predictive of peripheral blood stem cell yield and successful collection in healthy allogeneic donors

Background: An efficient mobilization and collection of peripheral blood stem cells (PBSCs) are crucial to optimize engraftment in the recipient. We aim to validate a formula that predicted CD34⁺ cell yield and to describe variables that correlated with high yield mobilization and collection in healthy donors.

Methods: We retrospectively analyzed clinical and laboratory data from healthy donors who underwent PBSC collection from 2006 to 2015. The predicted number of collected cells was calculated using the following formula: Total number of CD34⁺ (cells×10⁶/kg) yield=[(peripheral CD34⁺ cells/μL)×(0.43)/recipient body weight (kg)]×total liters processed.

Results: We evaluated 338 collections from 307 allogeneic PBSC donors. The predicted versus the observed number of CD34⁺ cells/kg collected yielded an r-value of 0.775 (0.726-0.816; p<0.0001). Overall, 55.7% donors had an acceptable mobilization level. Donors with a body weight <67kg were less likely to yield a satisfactory CD34⁺ cell count (OR=0.44; 95% CI 0.24-0.81), while a white blood cell (WBC) count >40×10⁹/L (OR=3.69; 2.11-6.46) and platelet count ≥200×10⁹/L (OR=2.09; 1.26-3.47) on the day of collection predicted a good level of mobilization. Predictors of a CD34⁺ cell yield/kg of ≥4×10⁶ with only one apheresis session were: circulating CD34⁺ cells/μL >40 (OR=16; 6.94-36.93), hemoglobin ≥14g/dL (OR=3.40; 1.53-7.57), WBC >40×10⁹/L (OR=4.61; 2.10-10.10) on the first collection day, and a positive delta weight between donor and recipient (OR=3.10; 1.36-7.06).

Conclusion: The formula for predicting CD34⁺ cell yield is accurate and suggests the optimal length of time for successful leukapheresis. Validation of the predictors of successful mobilization will help to further refine PBSC leukapheresis procedures.