Facile fully automated radiosynthesis and quality control of O-(2-[18F]fluoroethyl)-l-tyrosine ([18F]FET) for human brain tumor imaging

Appl Radiat Isot. 2019 Dec:154:108852. doi: 10.1016/j.apradiso.2019.108852. Epub 2019 Aug 12.

Abstract

O-(2-[18F]Fluoroethyl)-l-tyrosine ([18F]FET) has become one of the most successful amino acid tracers for human brain tumor imaging with positron emission tomography (PET). Facile fully automated radiosynthesis and quality control (QC) of [18F]FET using our home-built automated multi-purpose 18F-radiosynthesis module are described. [18F]FET was produced in 75-80 min overall synthesis time with 20-25% radiochemical yield decay corrected to end of bombardment (EOB), based on H[18F]F. The radiochemical and enantiomeric purities were >99%, and the molar activity (Am) was 189-411 GBq/μmol at EOB. The [18F]FET dose meets all QC criteria for clinical use, and is suitable for clinical PET study of brain tumor.

Keywords: Automation; Brain tumor; O-(2-[(18)F]Fluoroethyl)-(l)-tyrosine ([(18)F]FET); Positron emission tomography (PET); Quality control (QC); Radiosynthesis.

MeSH terms

  • Brain Neoplasms / diagnostic imaging*
  • Chromatography, High Pressure Liquid
  • Fluorine Radioisotopes* / chemistry
  • Humans
  • Positron-Emission Tomography / methods
  • Quality Control
  • Radiopharmaceuticals / chemical synthesis*
  • Radiopharmaceuticals / chemistry
  • Radiopharmaceuticals / standards
  • Stereoisomerism
  • Tyrosine / analogs & derivatives*
  • Tyrosine / chemical synthesis
  • Tyrosine / chemistry
  • Tyrosine / standards

Substances

  • Fluorine Radioisotopes
  • O-(2-fluoroethyl)tyrosine
  • Radiopharmaceuticals
  • Tyrosine