Abstract
Lymphokine production in human peripheral blood mononuclear cells (PBMNCs) was investigated by in situ hybridization with 35S-labelled RNA probes coding for interleukin 2 (IL-2), gamma-interferon (gamma-IFN) and granulocyte/macrophage colony stimulating factor (GM-CSF). The proportion of PBMNCs expressing lymphokine mRNA varied according to the stimuli used and followed similar patterns to those previously observed by Northern blot analysis. Only a relatively low proportion of PBMNCs expressed lymphokine mRNA after stimulation for a given time, and this seems not to be due to the heterogeneity of T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Colony-Stimulating Factors / biosynthesis
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Colony-Stimulating Factors / genetics
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Granulocyte-Macrophage Colony-Stimulating Factor
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Growth Substances / biosynthesis
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Growth Substances / genetics
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Humans
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Interferon-gamma / genetics
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Interleukin-2 / biosynthesis
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Interleukin-2 / genetics
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Lymphocytes / immunology*
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Lymphokines / biosynthesis*
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Lymphokines / genetics
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Nucleic Acid Hybridization
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RNA Probes
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
Substances
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Colony-Stimulating Factors
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Growth Substances
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Interleukin-2
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Lymphokines
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RNA Probes
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RNA, Messenger
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor