Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor

Musab M Aldhahri; Yaaser Q Almulaiky; Reda M El-Shishtawy; Waleed Al-Shawafi; Ahmed Alngadh; Rayan Maghrabi

doi:10.1021/acsomega.8b00366

Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor

ACS Omega. 2018 Jun 13;3(6):6346-6350. doi: 10.1021/acsomega.8b00366. eCollection 2018 Jun 30.

Authors

Musab M Aldhahri^{1

2}, Yaaser Q Almulaiky³, Reda M El-Shishtawy^{2

4}, Waleed Al-Shawafi², Ahmed Alngadh⁵, Rayan Maghrabi²

Affiliations

¹ Center of Nanotechnology, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
² Department of Biochemistry, Department of Chemistry, and Department of Biochemistry, King Abdulaziz University, P. O. Box 80200, Jeddah 21589, Saudi Arabia.
³ Department of Biochemistry, Faculty of Science, University of Jeddah, P.O.Box 80203, Jeddah 21589, Saudi Arabia.
⁴ Department of Dyeing, Printing and Textile Auxiliaries, National Research Centre, Dokki, 71516 Cairo, Egypt.
⁵ King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia.

Abstract

The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized α-amylases with regard to pH, thermal stability, and reusability were studied. An organic light emitting material tris(8-hydroxyquinoline)aluminum was incorporated to monitor the enzyme activity for several reuses.