Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Lu Qi; Aihong Zhang; Yu Wang; Long Liu; Xinghe Wang

doi:10.1186/s13065-019-0622-7

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

BMC Chem. 2019 Aug 22;13(1):110. doi: 10.1186/s13065-019-0622-7. eCollection 2019 Dec.

Authors

Lu Qi¹, Aihong Zhang², Yu Wang¹, Long Liu¹, Xinghe Wang¹

Affiliations

¹ 1Phase I Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038 China.
² Institute of Chemical Defense, Beijing, 102205 China.

Abstract

A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction.

Keywords: Atom transfer radical polymer; Enzyme-linked immunosorbent assay; Gold nanoparticles; Graphene oxide; Paper.