Next-generation sequencing specifies Angiostrongylus eosinophilic meningoencephalitis in infants: Two case reports

Medicine (Baltimore). 2019 Aug;98(35):e16985. doi: 10.1097/MD.0000000000016985.

Abstract

Rationale: Angiostrongylus cantonensis-induced eosinophilic meningoencephalitis (AEM) in infants is a very rare but fatal disease. Utilization of genetic assay to detect the cerebral parasite plays an important role for the treatment of the infection.

Patient concerns: Two infants (<2 years) presented with cough, intermittent fever, mental fatigue, and poor diet.

Diagnosis: The patients were under clinical examination and laboratory test including cardiac ultrasound, chest X-ray, blood or cerebrospinal fluid (CSF) cell counting, serum enzyme-linked immunosorbent assay (ELISA), head magnetic resonance imaging (MRI) and next-generation sequencing (NGS) on DNA from CSF. Due to hypereosinophils in patients' peripheral blood and CSF, and abundant DNA sequences from A cantonensis in CSF, the patients were diagnosed with Angiostrongylus eosinophilic meningoencephalitis.

Interventions: The patients were treated with albendazole to deworm, and methylprednisolone to reduce inflammation.

Outcome: The patients were completely recovered from AEM without relapse after 10-day treatment.

Lessons: ELISA and MRI are not sufficiently accurate for the diagnosis of AEM in infants. NGS can specify the infection by the cerebral parasite and offers a new effective approach for the early and precise diagnosis of AEM in infants.

Publication types

  • Case Reports

MeSH terms

  • Albendazole / therapeutic use
  • Angiostrongylus cantonensis
  • Animals
  • Anthelmintics / therapeutic use
  • Anti-Inflammatory Agents / therapeutic use
  • Enzyme-Linked Immunosorbent Assay
  • Eosinophilia / complications*
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Infant
  • Magnetic Resonance Imaging
  • Male
  • Meningoencephalitis / complications*
  • Meningoencephalitis / diagnosis*
  • Meningoencephalitis / drug therapy
  • Meningoencephalitis / parasitology*
  • Methylprednisolone / therapeutic use
  • Strongylida Infections / diagnosis*
  • Strongylida Infections / drug therapy
  • Strongylida Infections / parasitology

Substances

  • Anthelmintics
  • Anti-Inflammatory Agents
  • Albendazole
  • Methylprednisolone