Effectiveness of Perioperative Immunologic Markers Monitoring for Predicting Early Acute Cellular Rejection After Living Donor Liver Transplantation

Transplant Proc. 2019 Oct;51(8):2648-2654. doi: 10.1016/j.transproceed.2019.03.077. Epub 2019 Aug 30.

Abstract

Background: The objective of this study was to determine whether perioperative immunologic markers monitoring could predict early acute cellular rejection (ACR) after living donor liver transplantation (LDLT).

Materials and methods: From September 2010 to June 2013, a total of 172 patients underwent LDLT at our transplant center. Of them, 26 patients were excluded because of infection. We retrospectively reviewed the remaining 146 patients. CD4 lymphocyte activity, T cell subsets test, and serum cytokine panel were checked on the day before transplantation and at 20 days after transplantation. These patients were divided into 3 groups: 1. normal liver function test (LFT) group; 2. increased LFT without rejection group; and 3. early ACR group. We excluded the increased LFT without rejection group in order to rule out multiple factors influencing immunologic factors.

Results: CD4 lymphocyte activity (P = .004) was significantly increased while CD4+/CD25+/FOXP3+ cells (P < .001) and interleukin (IL)-17 (P = .002) levels were significantly decreased during the perioperative period. Pretransplant IL-6 (P = .014) and IL-17 (P = .029) levels in the early ACR group were significantly lower than those in the normal LFT group. The proportion of patients with increased IL-6 during perioperative period in the early ACR group was higher than that in the normal LFT group, although the difference was not statistically significant (P = .065).

Conclusion: Our results suggest that IL-6 and IL-17 levels are associated with early ACR in LDLT patients. However, whether monitoring perioperative immunologic markers could predict early ACR remains unclear. Further prospective studies are needed to reach a definite conclusion.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Biomarkers / blood
  • CD4-Positive T-Lymphocytes / immunology
  • Female
  • Graft Rejection / immunology*
  • Humans
  • Interleukin-17 / blood*
  • Interleukin-6 / blood*
  • Liver Transplantation / adverse effects*
  • Liver Transplantation / methods
  • Living Donors
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Preoperative Period
  • Prospective Studies
  • Retrospective Studies
  • T-Lymphocyte Subsets / immunology

Substances

  • Biomarkers
  • IL17A protein, human
  • Interleukin-17
  • Interleukin-6