Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene tet(X4)

Liang-Xing Fang; Chong Chen; Dong-Ling Yu; Ruan-Yang Sun; Chao-Yue Cui; Liang Chen; Xiao-Ping Liao; Ya-Hong Liu; Jian Sun

doi:10.1128/AAC.01373-19

Complete Nucleotide Sequence of a Novel Plasmid Bearing the High-Level Tigecycline Resistance Gene tet(X4)

Antimicrob Agents Chemother. 2019 Oct 22;63(11):e01373-19. doi: 10.1128/AAC.01373-19. Print 2019 Nov.

Authors

Liang-Xing Fang^#^{1

2}, Chong Chen^#^{1

2}, Dong-Ling Yu^#³, Ruan-Yang Sun^{1

2}, Chao-Yue Cui^{1

2}, Liang Chen⁴, Xiao-Ping Liao^{1

2}, Ya-Hong Liu^{1

2}, Jian Sun^{5

2}

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
³ The Teaching and Research Department of Biotechnology, Nanning University, Guangxi, China.
⁴ Hackensack-Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA.
⁵ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China [email protected].

^# Contributed equally.

Abstract

We reported the complete nucleotide sequence of a tet(X4)-carrying plasmid, pSTB20-1T, from a tigecycline-resistant Escherichia coli isolate in China. Sequence analysis indicated that pSTB20-1T contains a hybrid plasmid backbone and a tet(X4)-containing multidrug resistance region, likely originated through recombination of multiple plasmids. tet(X4) was flanked by two ISCR2, which may be responsible for tet(X4) mobilization. The occurrence and transmission of this novel hybrid plasmid may exacerbate the spread of the clinically significant tet(X4) gene.

Keywords: Escherichia coli; hybrid plasmid; tet(X4); tigecycline resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Drug Resistance, Bacterial / genetics
Escherichia coli / drug effects
Escherichia coli / genetics*
Genes, Bacterial / genetics*
Plasmids / genetics*
Sequence Analysis, DNA
Tigecycline / pharmacology*

Substances

Anti-Bacterial Agents
Tigecycline