Genetic voltage indicators

BMC Biol. 2019 Sep 12;17(1):71. doi: 10.1186/s12915-019-0682-0.

Abstract

As a "holy grail" of neuroscience, optical imaging of membrane potential could enable high resolution measurements of spiking and synaptic activity in neuronal populations. This has been partly achieved using organic voltage-sensitive dyes in vitro, or in invertebrate preparations yet unspecific staining has prevented single-cell resolution measurements from mammalian preparations in vivo. The development of genetically encoded voltage indicators (GEVIs) and chemogenetic sensors has enabled targeting voltage indicators to plasma membranes and selective neuronal populations. Here, we review recent advances in the design and use of genetic voltage indicators and discuss advantages and disadvantages of three classes of them. Although genetic voltage indicators could revolutionize neuroscience, there are still significant challenges, particularly two-photon performance. To overcome them may require cross-disciplinary collaborations, team effort, and sustained support by large-scale research initiatives.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Cell Membrane / metabolism
  • Fluorescent Dyes / chemistry*
  • Luminescent Proteins* / chemistry
  • Luminescent Proteins* / genetics
  • Neurons / physiology*
  • Rhodopsin / chemistry
  • Rhodopsin / genetics
  • Single-Cell Analysis / methods
  • Voltage-Dependent Anion Channels* / chemistry
  • Voltage-Dependent Anion Channels* / genetics

Substances

  • Fluorescent Dyes
  • Luminescent Proteins
  • Voltage-Dependent Anion Channels
  • Rhodopsin