Bacterial community structure analysis of a hot spring soil by next generation sequencing of ribosomal RNA

Nivedita Rawat; Gopal Krishna Joshi

doi:10.1016/j.ygeno.2018.06.008

Bacterial community structure analysis of a hot spring soil by next generation sequencing of ribosomal RNA

Genomics. 2019 Sep;111(5):1053-1058. doi: 10.1016/j.ygeno.2018.06.008. Epub 2018 Jul 2.

Authors

Nivedita Rawat¹, Gopal Krishna Joshi²

Affiliations

¹ Department of Zoology & Biotechnology, HNB Garhwal University, Srinagar(Garhwal), Uttarakhand, India.
² Department of Zoology & Biotechnology, HNB Garhwal University, Srinagar(Garhwal), Uttarakhand, India. Electronic address: [email protected].

PMID: 31533897
DOI: 10.1016/j.ygeno.2018.06.008

Abstract

In the present study the whole bacterial community structure of Tapovan hot spring soil located in the state of Uttarakhand, India was analysed through next generation sequencing. The hot spring soil is slightly alkaline in nature with abundance of sulphur. The spring soil was rich in various metallic and non-metallic elements required for bacterial survival. The community was found to comprise of 14 bacterial phyla with representation of members belonging to Firmicutes, Proteobacteria, Thermi, Bacteroidetes, Aquificae, Actinobacteria, chloroflexi, TM7, Fusobacteria etc. At the genus level Bacillus, Pseudomonas, Symbiobacterium, Thermus, Geobacillus, Anoxybacillus were found in abundance as compared to other genera like Flavobacterium, Ureibacillus, Clostridium, Meiothermus, Acinetobacter, Desulfotomaculum and Rheinheimera.

Keywords: Bacterial diversity; Hot spring soil; Metagenome; Next generation sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / classification
Bacteria / genetics*
Hot Springs / microbiology*
Metagenome*
Microbiota*
RNA, Ribosomal / genetics
Soil / chemistry
Soil Microbiology*

Substances

RNA, Ribosomal
Soil