Identification of the BRAF V600E mutation in a patient with sclerosing pneumocytoma: A case report

Lung Cancer. 2019 Nov:137:52-55. doi: 10.1016/j.lungcan.2019.09.004. Epub 2019 Sep 6.

Abstract

Objectives: Sclerosing pneumocytoma (sclerosing hemangioma, SP) is a rare benign tumor of the lung with a low risk of recurrence. The genomic profile of SP is not well-known. Here we report gene mutation findings in a 17-year-old girl with SP.

Materials and methods: Immunohistochemistry (IHC), next-generation sequencing (NGS), and sanger sequencing were performed on the tumor tissue of this patient for pathological diagnosis and gene mutation analysis.

Results and conclusion: Two mutations were identified in the tumor tissue by NGS and sanger sequencing: AKT1 E17K and BRAF (B-Raf proto-oncogene, serine/threonine kinase) V600E. This is the first case report of a BRAF V600E mutation in a patient with SP. This discovery extends our understanding of the pathogenesis of SP, and suggests the need for future testing of BRAF V600E in this rare tumor type.

Keywords: BRAF V600E; Benign tumor; Next-generation sequencing; Sclerosing pneumocytoma.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Biomarkers, Tumor / genetics*
  • DNA Mutational Analysis
  • Female
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Mutation*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins B-raf / genetics*
  • Pulmonary Sclerosing Hemangioma / genetics*
  • Pulmonary Sclerosing Hemangioma / pathology

Substances

  • Biomarkers, Tumor
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • BRAF protein, human
  • Proto-Oncogene Proteins B-raf