Advances in high-resolution microscopy for the study of intracellular interactions with biomaterials

Catherine S Hansel; Margaret N Holme; Sahana Gopal; Molly M Stevens

doi:10.1016/j.biomaterials.2019.119406

Advances in high-resolution microscopy for the study of intracellular interactions with biomaterials

Biomaterials. 2020 Jan:226:119406. doi: 10.1016/j.biomaterials.2019.119406. Epub 2019 Aug 6.

Authors

Catherine S Hansel¹, Margaret N Holme², Sahana Gopal³, Molly M Stevens⁴

Affiliations

¹ Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London, UK; Department of Chemistry, Imperial College London, London, UK.
² Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
³ Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London, UK; Department of Medicine, Imperial College London, London, UK.
⁴ Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London, UK; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden. Electronic address: [email protected].

PMID: 31558349
DOI: 10.1016/j.biomaterials.2019.119406

Abstract

The study of sophisticated biomaterials and their cellular targets requires visualization methods with exquisite spatial and temporal resolution to discern cell organelles and molecular events. Monitoring cell-material interactions at high resolution is key for the continued development and optimization of biomaterials, for monitoring cell uptake of cargo, and for understanding the cell response to extracellular cues. This review evaluates the advantages and disadvantages of different forms of electron microscopy and super-resolution microscopy in elucidating how biomaterial surface chemistry and topography can affect intracellular events at the nanoscale.

Keywords: Cell-material interactions; Electron microscopy; Super-resolution microscopy.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biocompatible Materials*
Microscopy, Electron
Organelles*

Substances

Biocompatible Materials

Abstract

Publication types

MeSH terms

Substances

Grants and funding