Rationally Engineered Nucleic Acid Architectures for Biosensing Applications

Chem Rev. 2019 Nov 27;119(22):11631-11717. doi: 10.1021/acs.chemrev.9b00121. Epub 2019 Oct 1.

Abstract

Development of biosensing platforms plays a key role in research settings for identification of biomarkers and in clinical applications for diagnostics. Biosensors based on nucleic acids have taken many forms, from simple duplex-based constructs to stimuli-responsive nucleic acid nanostructures. In this review, we look at various nucleic acid-based biosensors, the different read-out strategies employed, and their use in chemical and biological sensing. We also look at current developments in DNA nanotechnology-based biosensors and how rational design of such constructs leads to more efficient biosensing platforms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • DNA / chemistry
  • Fluorescence Resonance Energy Transfer / methods
  • Humans
  • Models, Molecular
  • Nucleic Acid Conformation
  • Nucleic Acids / chemistry*

Substances

  • Nucleic Acids
  • DNA