Spider Chitin. The biomimetic potential and applications of Caribena versicolor tubular chitin

Tomasz Machałowski; Marcin Wysokowski; Sonia Żółtowska-Aksamitowska; Nicole Bechmann; Björn Binnewerg; Mario Schubert; Kaomei Guan; Stefan R Bornstein; Katarzyna Czaczyk; Oleg Pokrovsky; Michael Kraft; Martin Bertau; Christian Schimpf; David Rafaja; Mikhail Tsurkan; Roberta Galli; Heike Meissner; Iaroslav Petrenko; Andriy Fursov; Alona Voronkina; Marek Figlerowicz; Yvonne Joseph; Teofil Jesionowski; Hermann Ehrlich

doi:10.1016/j.carbpol.2019.115301

Spider Chitin. The biomimetic potential and applications of Caribena versicolor tubular chitin

Carbohydr Polym. 2019 Dec 15:226:115301. doi: 10.1016/j.carbpol.2019.115301. Epub 2019 Sep 8.

Authors

Tomasz Machałowski¹, Marcin Wysokowski², Sonia Żółtowska-Aksamitowska², Nicole Bechmann³, Björn Binnewerg⁴, Mario Schubert⁴, Kaomei Guan⁴, Stefan R Bornstein⁵, Katarzyna Czaczyk⁶, Oleg Pokrovsky⁷, Michael Kraft⁸, Martin Bertau⁸, Christian Schimpf⁹, David Rafaja⁹, Mikhail Tsurkan¹⁰, Roberta Galli¹¹, Heike Meissner¹², Iaroslav Petrenko¹³, Andriy Fursov¹³, Alona Voronkina¹⁴, Marek Figlerowicz¹⁵, Yvonne Joseph¹³, Teofil Jesionowski¹⁶, Hermann Ehrlich¹⁷

Affiliations

¹ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, Poland.
² Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, Poland; Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Freiberg 09599, Germany.
³ Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, TU Dresden, Dresden 01307, Germany.
⁴ Institute of Pharmacology and Toxicology, TU Dresden, Dresden 01307, Germany.
⁵ Department of Medicine III, University Hospital Carl Gustav Carus Dresden, TU Dresden, Dresden 01307, Germany.
⁶ Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznan 60637, Poland.
⁷ Geoscience and Environment Toulouse, UMR 5563 CNRS, Toulouse 31400, France; BIO-GEO-CLIM Laboratory, Tomsk State University, Tomsk, Russia.
⁸ Institute of Chemical Technology, TU Bergakademie Freiberg, Freiberg 09599, Germany.
⁹ Institute of Materials Science, TU Bergakademie Freiberg, Freiberg 09599, Germany.
¹⁰ Leibnitz Institute of Polymer Research Dresden, Dresden 01069, Germany.
¹¹ Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden 01307, Germany.
¹² Department of Prosthetic Dentistry, Faculty of Medicine, TU Dresden, Dresden 01307, Germany.
¹³ Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Freiberg 09599, Germany.
¹⁴ Department of Pharmacy, National Pirogov Memorial Medical University, Vinnytsia 21018, Ukraine.
¹⁵ Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan 61704, Poland.
¹⁶ Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Poznan 60965, Poland. Electronic address: [email protected].
¹⁷ Institute of Electronics and Sensor Materials, TU Bergakademie Freiberg, Freiberg 09599, Germany. Electronic address: [email protected].

PMID: 31582063
DOI: 10.1016/j.carbpol.2019.115301

Abstract

Diverse fields of modern technology and biomedicine can benefit from the application of ready-to-use chitin-based scaffolds. In this work we show for the first time the applicability of tubular and porous chitin from Caribena versicolor spiders as a scaffold for the development of an effective CuO/Cu(OH)₂ catalyst for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AM), and as a scaffold for the tissue engineering of selected cells. The formation of CuO/Cu(OH)₂ phases on and within the chitinous tubes leads to a hybrid material with excellent catalytic performance with respect to the reduction of p-nitrophenol. On the other hand, experimental results provide for the first time strong evidence for the biocompatibility of spider chitin with different cell types, a human progenitor cell line (hPheo1), as well as cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs) that were cultured on a tube-like scaffold.

Keywords: biocompatibility; biopolymers; catalyst; chitin; human cells; spider molt cuticle; tissue engineering.

MeSH terms

Animals
Arachnida / metabolism*
Biomimetic Materials / chemistry*
Cells, Cultured
Chitin / chemistry*
Humans
Tissue Engineering*
Tissue Scaffolds / chemistry*

Substances

Chitin