Eleven High-Quality Reference Genome Sequences and 360 Draft Assemblies of Shiga Toxin-Producing Escherichia coli Isolates from Human, Food, Animal, and Environmental Sources in Canada

Shari Tyson; Christy-Lynn Peterson; Adam Olson; Shaun Tyler; Natalie Knox; Emma Griffiths; Damion Dooley; William Hsiao; Jennifer Cabral; Roger P Johnson; Chad Laing; Victor Gannon; Tarah Lynch; Gary Van Domselaar; Fiona Brinkman; Morag Graham

doi:10.1128/MRA.00625-19

Eleven High-Quality Reference Genome Sequences and 360 Draft Assemblies of Shiga Toxin-Producing Escherichia coli Isolates from Human, Food, Animal, and Environmental Sources in Canada

Microbiol Resour Announc. 2019 Oct 10;8(41):e00625-19. doi: 10.1128/MRA.00625-19.

Authors

Shari Tyson^#¹, Christy-Lynn Peterson^#¹, Adam Olson^#¹, Shaun Tyler¹, Natalie Knox^{1

2}, Emma Griffiths^{3

4}, Damion Dooley⁴, William Hsiao⁴, Jennifer Cabral¹, Roger P Johnson⁵, Chad Laing⁶, Victor Gannon⁶, Tarah Lynch¹, Gary Van Domselaar^{1

2}, Fiona Brinkman³, Morag Graham^{7

2}

Affiliations

¹ National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
² Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
³ Department of Molecular Biology and Biochemistry, Simon Fraser University, Vancouver, British Columbia, Canada.
⁴ British Columbia Public Health Microbiology and Reference Laboratory, Vancouver, British Columbia, Canada.
⁵ National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.
⁶ National Microbiology Laboratory, Public Health Agency of Canada, Lethbridge, Alberta, Canada.
⁷ National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada [email protected].

^# Contributed equally.

Abstract

We report high-quality closed reference genomes for 1 bovine strain and 10 human Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from serogroups O26, O45, O91, O103, O104, O111, O113, O121, O145, and O157. We also report draft assemblies, with standardized metadata, for 360 STEC strains isolated from watersheds, animals, farms, food, and human infections.