Adult sox10+ Cardiomyocytes Contribute to Myocardial Regeneration in the Zebrafish

Marcos Sande-Melón; Inês J Marques; María Galardi-Castilla; Xavier Langa; María Pérez-López; Marius-Alexandru Botos; Héctor Sánchez-Iranzo; Gabriela Guzmán-Martínez; David Miguel Ferreira Francisco; Dinko Pavlinic; Vladimir Benes; Rémy Bruggmann; Nadia Mercader

doi:10.1016/j.celrep.2019.09.041

Adult sox10⁺ Cardiomyocytes Contribute to Myocardial Regeneration in the Zebrafish

Cell Rep. 2019 Oct 22;29(4):1041-1054.e5. doi: 10.1016/j.celrep.2019.09.041.

Affiliations

¹ Spanish National Centre for Cardiovascular Research Carlos III (CNIC-ISCIII), 28029 Madrid, Spain; Institute of Anatomy, University of Bern, 3012 Bern, Switzerland.
² Institute of Anatomy, University of Bern, 3012 Bern, Switzerland.
³ Spanish National Centre for Cardiovascular Research Carlos III (CNIC-ISCIII), 28029 Madrid, Spain.
⁴ Spanish National Centre for Cardiovascular Research Carlos III (CNIC-ISCIII), 28029 Madrid, Spain; Departamento de Cardiología, Hospital Universitario La Paz. IdiPaz, 28046 Madrid, Spain.
⁵ Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, 3012 Bern, Switzerland.
⁶ Genomics Core Facility, European Molecular Biology Laboratory (EMBL) Heidelberg, 69117 Heidelberg, Germany.
⁷ Spanish National Centre for Cardiovascular Research Carlos III (CNIC-ISCIII), 28029 Madrid, Spain; Institute of Anatomy, University of Bern, 3012 Bern, Switzerland. Electronic address: [email protected].

Abstract

During heart regeneration in the zebrafish, fibrotic tissue is replaced by newly formed cardiomyocytes derived from preexisting ones. It is unclear whether the heart is composed of several cardiomyocyte populations bearing different capacity to replace lost myocardium. Here, using sox10 genetic fate mapping, we identify a subset of preexistent cardiomyocytes in the adult zebrafish heart with a distinct gene expression profile that expanded after cryoinjury. Genetic ablation of sox10⁺ cardiomyocytes impairs cardiac regeneration, revealing that these cells play a role in heart regeneration.

Keywords: cardiomyocyte proliferation; heart regeneration; sox10; zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Proliferation
Cells, Cultured
Heart / physiology
Myocytes, Cardiac / metabolism*
Myocytes, Cardiac / physiology
Regeneration*
SOXE Transcription Factors / genetics
SOXE Transcription Factors / metabolism*
Zebrafish
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*

Substances

SOXE Transcription Factors
Zebrafish Proteins
sox10 protein, zebrafish