Optical properties of brain tissues at the different stages of glioma development in rats: pilot study

Elina A Genina; Alexey N Bashkatov; Daria K Tuchina; Polina A Dyachenko Timoshina; Nikita Navolokin; Alexander Shirokov; Alexander Khorovodov; Andrey Terskov; Maria Klimova; Aysel Mamedova; Inna Blokhina; Ilana Agranovich; Ekaterina Zinchenko; Oxana V Semyachkina-Glushkovskaya; Valery V Tuchin

doi:10.1364/BOE.10.005182

Optical properties of brain tissues at the different stages of glioma development in rats: pilot study

Biomed Opt Express. 2019 Sep 16;10(10):5182-5197. doi: 10.1364/BOE.10.005182. eCollection 2019 Oct 1.

Authors

Elina A Genina^{1

2}, Alexey N Bashkatov^{1

2}, Daria K Tuchina^{1

2

3}, Polina A Dyachenko Timoshina^{1

2}, Nikita Navolokin⁴, Alexander Shirokov⁵, Alexander Khorovodov¹, Andrey Terskov¹, Maria Klimova¹, Aysel Mamedova¹, Inna Blokhina¹, Ilana Agranovich¹, Ekaterina Zinchenko¹, Oxana V Semyachkina-Glushkovskaya¹, Valery V Tuchin^{1

2

6}

Affiliations

¹ Saratov State University, 83 Astrakhanskaya Str., Saratov 410012, Russia.
² Tomsk State University, 36 Lenin Avenue, Tomsk 634050, Russia.
³ Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova str., Moscow 119991, Russia.
⁴ Saratov State Medical University, 112, B. Kazachya str., Saratov 410012, Russia.
⁵ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, IBPPM RAS, 13 Prospekt Entuziastov, Saratov 410049, Russia.
⁶ Institute of Precision Mechanics and Control of the Russian Academy of Sciences, 24, Rabochaya Str., Saratov 410028, Russia.

Abstract

In this paper, measurements of the optical properties (diffuse reflectance, total and collimated transmittance) of brain tissues in healthy rats and rats with C6-glioma were performed in the spectral range from 350 to 1800 nm. Using these measurements, characteristic tissue optical parameters, such as absorption coefficient, scattering coefficient, reduced scattering coefficient, and scattering anisotropy factor were reconstructed. It was obtained that the 10-day development of glioma led to increase of absorption coefficient, which was associated with the water content elevation in the tumor. However, further development of the tumor (formation of the necrotic core) led to decrease in the water content. The dependence of the scattering properties on the different stages of model glioma development was more complex. Light penetration depth into the healthy and tumor brain was evaluated.