Denoising of diffusion MRI improves peripheral nerve conspicuity and reproducibility

Background: Quantitative diffusion MRI is a promising technique for evaluating peripheral nerve integrity but low signal-to-noise ratio (SNR) can impede measurement accuracy.

Purpose: To evaluate principal component analysis (PCA) and generalized spherical deconvolution (genSD) denoising techniques to improve within-subject reproducibility and peripheral nerve conspicuity.

Study type: Prospective.

Subjects: Seven healthy volunteers and three peripheral neuropathy patients.

Field strength/sequence: 3T/multiband single-shot echo planar diffusion sequence using multishell 55-direction scheme.

Assessment: Images were processed using four methods: "original" (no denoising), "average" (10 repetitions), "PCA-only," and "PCA + genSD." Tibial and common peroneal nerve segmentations and masks were generated from volunteer diffusion data. Quantitative (SNR and contrast-to-noise ratio [CNR]) values were calculated. Three radiologists qualitatively evaluated nerve conspicuity for each method. The two denoising methods were also performed in three patients with peripheral neuropathies.

Statistical tests: For healthy volunteers, calculations included SNR and CNR_FA (computed using FA values). Coefficient of variation (CV%) of CNR_FA quantified within-subject reproducibility. Groups were compared with two-sample t-tests (significance P < 0.05; two-tailed, Bonferroni-corrected). Odds ratios (ORs) quantified the relative rates of each of three radiologists confidently identifying a nerve, per slice, for the four methods.

Results: "PCA + genSD" yielded the highest SNR (mean_overall = 14.83 ± 1.99) and tibial and common peroneal nerve CNR_FA (mean_tibial = 3.45, mean_peroneal = 2.34) compared to "original" (P _SNR < 0.001; P _CNR = 0.011) and "PCA-only" (P _SNR < 0.001, P _CNR < 0.001). "PCA + genSD" had higher within-subject reproducibility (low CV%) for tibial (6.04 ± 1.98) and common peroneal nerves (8.27 ± 2.75) compared to "original" and "PCA-only." The mean FA was higher for "original" than "average" (P < 0.001), but did not differ significantly between "average" and "PCA + genSD" (P = 0.14). "PCA + genSD" had higher tibial and common peroneal nerve conspicuity than "PCA-only" (OR_tibial = 2.50, P < 0.001; OR_peroneal = 1.86, P < 0.001) and "original" (OR_tibial = 2.73, P < 0.001; OR_peroneal = 2.43, P < 0.001).

Data conclusion: PCA + genSD denoising method improved SNR, CNR_FA , and within-subject reproducibility (CV%) without biasing FA and nerve conspicuity. This technique holds promise for facilitating more reliable, unbiased diffusion measurements of peripheral nerves.

Level of evidence: 2 Technical Efficacy Stage: 1 J. Magn. Reson. Imaging 2020;51:1128-1137.