Enhanced scale and scope of genome engineering and regulation using CRISPR/Cas in Saccharomyces cerevisiae

Matthew Deaner; Hal S Alper

doi:10.1093/femsyr/foz076

Enhanced scale and scope of genome engineering and regulation using CRISPR/Cas in Saccharomyces cerevisiae

FEMS Yeast Res. 2019 Nov 1;19(7):foz076. doi: 10.1093/femsyr/foz076.

Authors

Matthew Deaner¹, Hal S Alper^{1

2}

Affiliations

¹ McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, USA.
² Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, USA.

PMID: 31665284
DOI: 10.1093/femsyr/foz076

Abstract

Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this minireview, we discuss fundamental CRISPR technologies, tools to improve the efficiency and capabilities of gene targeting, and cutting-edge techniques to explore gene editing and transcriptional regulation at genome scale using pooled approaches. The focus is on applications to metabolic engineering with topics including development of techniques to edit the genome in multiplex, tools to enable large numbers of genetic modifications using pooled single-guide RNA libraries and efforts to enable programmable transcriptional regulation using endonuclease-null Cas enzymes.

Keywords: Saccharomyces cerevisiae; CRISPR; Cas9; Cpf1; dCas9; metabolic engineering; synthetic biology.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Endonucleases / genetics
Gene Expression Regulation, Fungal
Genetic Engineering / methods*
Genome, Fungal*
Metabolic Engineering
RNA, Guide, CRISPR-Cas Systems / genetics
Saccharomyces cerevisiae / genetics*
Synthetic Biology

Substances

RNA, Guide, CRISPR-Cas Systems
Endonucleases