Traumatic Ulcerative Granuloma with Stromal Eosinophilia: CD30 analysis and clonality for T cell receptor gene re-arrangement

Acta Histochem. 2019 Nov;121(8):151450. doi: 10.1016/j.acthis.2019.151450. Epub 2019 Oct 29.

Abstract

Introduction: Traumatic Ulcerative Granuloma with Stromal Eosinophilia (TUGSE) is a rare oral ulcerated lesion of uncertain etiology, showing eosinophil-rich granulation tissue, with occasional large atypical CD30 positive mononuclear cells. It had been suggested that it may represent an oral counterpart of cutaneous lymphomatoid papulosis, with a potential to evolve into CD30 + T cell lymphoma OBJECTIVES: To compare TUGSE and non-specific oral ulcers (NSU) clinically, histopathologically and by clonality analysis for T-cell receptor re-arrangement, aiming to determine whether TGUSE with atypical cells is a lymphomatous premalignant condition, and whether therapeutic approach should be radical or conservative.

Materials and methods: Retrospective archival analysis included 17 TUGSE and 8 NSU cases. Histopathological parameters included mean eosinophil number per high power field (HPF), presence of infiltration of deep soft tissues and presence of atypical cells. Immuno-morphometry comprised of the mean number of CD30+ atypical cells per HPF. T-cell receptor (TCR) gene rearrangement by polymerase chain reaction (PCR) was performed in all cases showing atypical cells. Clinical and follow up data were retrieved from files.

Results: TUGSE showed a significantly higher mean eosinophil number/HPF in comparison to NSU (7.0 + 4.2 cells and 2.3 + 1.72, respectively; p < 0.001). Atypical cells were found in 9 (53%) cases of TUGSE and in only 1 (11%) case of NSU. CD30+ atypical cells were found in 7 (41%) cases of TUGSE and only in 1 (11%) case of NSU. Mean number of CD30+ cells/HPF was 0.23 + 0.19 (range 0 - 0.54 cells/HPF) for TUGSE. In the only NSU case with CD30+ cells, their density was 0.52/HPF. All lesions with atypical cells were polyclonal for TCR. All cases were self-limiting, with no recurrences, after 3-9 years (mean 4.6 years) follow up.

Conclusions: Analysis found no support to the suggestion that TUGSE with atypical cells represents the oral counterpart of lymphomatoid papulosis or predisposes the lesions for a hematolymphoid malignancy. Suggestions for radical therapeutic approach and long-term follow-up are probably unjustified, with no recurrences or malignancy recorded following conservative treatment alone for a period of up to 9 years of follow-up. Staining for CD30 and PCR for TCR gene rearrangement should be reserved only for rare cases with abundant large atypical cells and/or unusual clinical behavior.

Keywords: CD30; Clonality; Eosinophilia; Ulcerative granuloma.

Publication types

  • Case Reports
  • Clinical Trial

MeSH terms

  • Aged
  • Aged, 80 and over
  • Child
  • Eosinophilia / genetics
  • Eosinophilia / metabolism
  • Eosinophilia / pathology
  • Female
  • Follow-Up Studies
  • Gene Rearrangement, T-Lymphocyte*
  • Granuloma* / genetics
  • Granuloma* / metabolism
  • Granuloma* / pathology
  • Humans
  • Ki-1 Antigen* / genetics
  • Ki-1 Antigen* / metabolism
  • Lymphomatoid Granulomatosis* / genetics
  • Lymphomatoid Granulomatosis* / metabolism
  • Lymphomatoid Granulomatosis* / pathology
  • Male
  • Middle Aged
  • Mouth Neoplasms* / genetics
  • Mouth Neoplasms* / metabolism
  • Mouth Neoplasms* / pathology
  • Neoplasm Proteins* / genetics
  • Neoplasm Proteins* / metabolism
  • Oral Ulcer* / genetics
  • Oral Ulcer* / metabolism
  • Oral Ulcer* / pathology
  • Retrospective Studies
  • Wounds and Injuries* / genetics
  • Wounds and Injuries* / metabolism
  • Wounds and Injuries* / pathology

Substances

  • Ki-1 Antigen
  • Neoplasm Proteins