The sensing of protein is of great importance because of its prominent role in building and repairing tissues. In this work, we present a simple design for the detection and sensing of protein using one-dimensional defective photonic crystals. The main idea of our work is included in the theoretical investigation of the transmittance properties of the resonant mode produced inside the photonic band gap. Our study uses the characteristic matrix method and curve fitting. The main reason for our study is to detect the concentration of a protein solution using an efficient, accurate, and simple method. Here, the defect layer is filled with a protein solution. Our idea depends on two hypotheses, and the first one is based on the appearance of a resonant peak on the photonic band gap. The second one depends on a change in the position of this resonant peak with the concentration of the protein solution. The effect of many parameters on the performance of our sensor, such as the thickness of the defect layer and the sensitivity, is demonstrated. The numerical results could present a simple way to design an accurate, stable, efficient, and low-cost protein sensor compared to other current methods and techniques.