Interleukin-33 activates and recruits natural killer cells to inhibit pulmonary metastatic cancer development

Int J Cancer. 2020 Mar 1;146(5):1421-1434. doi: 10.1002/ijc.32779. Epub 2019 Dec 5.

Abstract

Increasing evidence suggests that IL-33 plays an important role in regulating tumor development. However, conflicting results, obtained from numerous studies, have highlighted the divergent functions of IL-33. The detailed mechanisms by which IL-33 modulates tumor development merit further investigation. Here, we report that IL-33 administration can effectively inhibit the development of pulmonary metastasis of breast cancer in a mouse. In our model, IL-33 promotes the production of TNF-α by macrophages, which increases IL-33 specific receptor (ST2) expression on natural killer (NK) cells and is pivotal in IL-33-induced NK cell activation. IL-33 treatment also facilitates the production of CCL5 in the lung by eosinophils and CD8+ T cells, which mediates the recruitment of NK cells to the tumor microenvironment. The systemic activation and local recruitment of NK cells result in potent tumor rejection in the lung. Our study reports a novel mechanism for the IL-33-meditated suppression of metastatic cancer and provides potential therapeutic strategies for targeting metastatic tumor.

Keywords: CCL5; IL-33; NK cells; TNF-α; metastatic cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / immunology*
  • Breast Neoplasms / mortality
  • Cell Line, Tumor
  • Computational Biology
  • Datasets as Topic
  • Disease Models, Animal
  • Female
  • Humans
  • Interleukin-1 Receptor-Like 1 Protein / metabolism
  • Interleukin-33 / administration & dosage
  • Interleukin-33 / immunology*
  • Kaplan-Meier Estimate
  • Killer Cells, Natural
  • Lung Neoplasms / immunology
  • Lung Neoplasms / prevention & control*
  • Lung Neoplasms / secondary
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology*
  • Macrophages / drug effects
  • Macrophages / immunology
  • Macrophages / metabolism
  • Mice
  • Primary Cell Culture
  • Prognosis
  • Recombinant Proteins / administration & dosage
  • Signal Transduction / drug effects
  • Signal Transduction / immunology
  • Survival Rate
  • Tumor Microenvironment / drug effects
  • Tumor Microenvironment / immunology*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • IL33 protein, human
  • Il1rl1 protein, mouse
  • Interleukin-1 Receptor-Like 1 Protein
  • Interleukin-33
  • Recombinant Proteins
  • Tnf protein, mouse
  • Tumor Necrosis Factor-alpha