In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

Diana Caridha; Susan Leed; Alicia Cawlfield

doi:10.1007/978-1-4939-9940-8_7

In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

Methods Mol Biol. 2020:2081:81-106. doi: 10.1007/978-1-4939-9940-8_7.

Authors

Diana Caridha¹, Susan Leed², Alicia Cawlfield³

Affiliations

¹ Walter Reed Army Institute of Research, Silver Spring, MD, USA. [email protected].
² Walter Reed Army Institute of Research, Silver Spring, MD, USA.
³ Dwight D. Eisenhower Army Medical Center, Fort Gordon, GA, USA.

PMID: 31721120
DOI: 10.1007/978-1-4939-9940-8_7

Abstract

Confirming the in vivo efficacy of potential antileishmanial compounds that display in vitro potency and good chemical characteristics is one of the most important steps in preclinical research drug discovery before human clinical trials begin. Here we describe the use of the in vivo bioluminescent monitoring of high and low inocula of luciferase-expressing Leishmania major (L. major) parasites in traditional and more innovative rodent models of in vivo cutaneous leishmaniasis (CL) drug discovery.

Keywords: Base of the tail; Bioluminescence signal; Cutaneous leishmaniasis (CL); Drug efficacy; Ear infections; Footpad; IVIS; In vivo imaging; Luciferase-expressing Leishmania major; Rodent models.

MeSH terms

Animals
Antiprotozoal Agents / pharmacology*
Disease Models, Animal
Drug Discovery / methods*
Drug Evaluation, Preclinical / methods*
Female
Gene Expression
Genes, Reporter
Leishmania major / drug effects*
Leishmaniasis, Cutaneous / drug therapy
Leishmaniasis, Cutaneous / parasitology*
Luminescent Measurements / methods*
Mice
Mice, Inbred BALB C

Substances

Antiprotozoal Agents