Two iPSC lines generated from the bone marrow of a relapsed/refractory AML patient display normal karyotypes and myeloid differentiation potential

Stem Cell Res. 2019 Dec:41:101587. doi: 10.1016/j.scr.2019.101587. Epub 2019 Oct 17.

Abstract

Using iPSCs to study cancer has been complicated by the fact that many cancer cells are difficult to reprogram, which has been attributed to the genomic abnormalities present. Acute Myeloid Leukemia (AML) is a complex disease that presents with various types of genomic aberrations that affect prognosis. Here we reprogrammed CD34+ cells from an AML patient containing a rare der(7)t(7;13) translocation associated with poor prognosis, who had relapsed and was refractory to current treatments. The generated AML-iPSCs displayed normal karyotypes and myeloid differentiation potential. These findings have implications for modeling and treating AML disease.

Keywords: Acute Myeloid Leukemia; Bone marrow cells; Disease modeling; Induced pluripotent stem cells; Reprogramming.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Bone Marrow / pathology*
  • Cell Differentiation*
  • Drug Resistance, Neoplasm*
  • Humans
  • Induced Pluripotent Stem Cells / pathology*
  • Karyotype
  • Leukemia, Myeloid, Acute / pathology*
  • Male
  • Myeloid Cells / pathology*
  • Neoplasm Recurrence, Local / pathology*
  • Tumor Cells, Cultured