Turn-on fluorescence and photodynamic activity of β-(1,3-1,6)-d-glucan-complexed porphyrin derivatives inside HeLa cells

Photochem Photobiol Sci. 2019 Dec 1;18(12):2854-2858. doi: 10.1039/c9pp00208a. Epub 2019 Nov 25.

Abstract

Fluorescence intensities of water-soluble β-(1,3-1,6)-d-glucan (β-1,3-glucan)-complexed porphyrin derivatives were very weak as a result of self-quenching. However, β-1,3-glucan-complexed tetra(aminophenyl)porphyrin exhibited 'off-state' to 'on-state' fluorescence switching activity by intracellular uptake. Furthermore, the internalised complex showed a high level of photodynamic activity toward HeLa cells under photoirradiation at long wavelengths.

MeSH terms

  • Glucans / chemistry*
  • HeLa Cells
  • Humans
  • Light
  • Microscopy, Fluorescence
  • Photochemotherapy
  • Porphyrins / chemistry*
  • Porphyrins / metabolism

Substances

  • Glucans
  • Porphyrins