MiR-3175 promotes epithelial-mesenchymal transition by targeting Smad7 in human conjunctiva and pterygium

FEBS Lett. 2020 Apr;594(7):1207-1217. doi: 10.1002/1873-3468.13698. Epub 2019 Dec 16.

Abstract

MicroRNAs (miRNAs) are involved in the post-transcriptional regulation of mRNAs. However, the function of miRNAs is unclear in epithelial-mesenchymal transition (EMT) of pterygium, a disease characterized by abnormal fibrovascular proliferation and invasion on the ocular surface. Since MiR-3175 is upregulated in pterygium tissues compared to normal conjunctival tissues, in this study, we investigated the role of MiR-3175 in EMT of a cultured human conjunctival epithelium cell line (CCL) and further validated the findings in pterygium tissues from patients. Our study demonstrates that MiR-3175 promotes proliferation, migration, invasion, and EMT of CCL cells by directly inhibiting Smad7. In conclusion, our data suggested that the MiR-3175-Smad7 axis may play a critical role in pathogenesis and development of pterygium.

Keywords: EMT; MiR-3175; Smad7; conjunctiva; pterygium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Movement
  • Cell Proliferation
  • Conjunctiva / metabolism*
  • Conjunctiva / pathology*
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Epithelial-Mesenchymal Transition / genetics*
  • Humans
  • MicroRNAs / genetics*
  • Pterygium / genetics*
  • Pterygium / pathology*
  • Smad7 Protein / genetics*
  • Smad7 Protein / metabolism
  • Vimentin / genetics

Substances

  • MIRN3175 microRNA, human
  • MicroRNAs
  • SMAD7 protein, human
  • Smad7 Protein
  • Vimentin