Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)

Nora Drick; Anais Sahabian; Praeploy Pongpamorn; Sylvia Merkert; Gudrun Göhring; Tobias Welte; Ulrich Martin; Ruth Olmer

doi:10.1016/j.scr.2019.101659

Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)

Stem Cell Res. 2020 Jan:42:101659. doi: 10.1016/j.scr.2019.101659. Epub 2019 Nov 19.

Authors

Nora Drick¹, Anais Sahabian², Praeploy Pongpamorn², Sylvia Merkert², Gudrun Göhring³, Tobias Welte⁴, Ulrich Martin², Ruth Olmer⁵

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Department of Pneumology and German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
² Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany.
³ Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.
⁴ Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Department of Pneumology and German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
⁵ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany. Electronic address: [email protected].

PMID: 31791010
DOI: 10.1016/j.scr.2019.101659

Abstract

Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells.

MeSH terms

Cell Differentiation
Cell Line
Humans
Induced Pluripotent Stem Cells / metabolism*
Thyroid Nuclear Factor 1 / genetics*
Transfection

Substances

NKX2-1 protein, human
Thyroid Nuclear Factor 1