Tri-ortho-cresyl phosphate (TOCP), a widely used plasticizer in industry, can cause female reproductive damage. Tea polyphenols (TPs) have multiple health effects via inhibiting oxidative stress. However, the reproductive protection of TPs in TOCP-induced female reproductive system damage is yet to be elucidated. In the study, TOCP inhibited cell viability and induced autophagy of mouse ovarian granulosa cells; while TPs could rescue the inhibition of viability and induction of autophagy. 3-MA, an autophagy inhibitor, could also rescue the inhibition of cell viability. These results indicated that TPs played a protective role in TOCP-induced autophagy. Furthermore, TPs could inhibit the induction of oxidative stress of the cells by TOCP, which implying that TPs might alleviate TOCP-induced autophagy via inhibiting oxidative stress. Furthermore, TPs could rescue TOCP-induced autophagy and oxidative stress in the mouse ovarian tissues. Taken together, these results indicated that TPs could protect TOCP-induced ovarian damage via inhibiting oxidative stress.
Keywords: autophagy; granulosa cells; oxidative stress; tea polyphenols; tri-ortho-cresyl phosphate.
© 2019 Wiley Periodicals, Inc.