The emergence of Staphylococcus epidermidis simultaneously nonsusceptible to linezolid and teicoplanin in China

Diagn Microbiol Infect Dis. 2020 Feb;96(2):114956. doi: 10.1016/j.diagmicrobio.2019.114956. Epub 2019 Nov 26.

Abstract

Two linezolid-resistant and teicoplanin-intermediate Staphylococcus epidermidis strains were isolated from blood cultures in China. The 2 S. epidermidis strains were methicillin-resistant and showed multidrug-resistance patterns; in addition, population analysis profiling/area under the curve (PAP/AUC) result showed heterogeneous resistant to vancomycin. Comparing to teicoplanin susceptible strains, the 2 isolates showed reduced autolytic activity. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) indicated that the 2 S. epidermidis isolates belonged to the same clone. Furthermore, the cfr gene, a G2576T mutation, and a novel C2146T mutation were detected in the 2 isolates. This was the first report of S. epidermidis simultaneously nonsusceptible to linezolid and teicoplanin in China.

Keywords: Linezolid-resistant; Staphylococcus epidermidis; Teicoplanin-intermediate; cfr gene.

MeSH terms

  • Adult
  • Alleles
  • Anti-Bacterial Agents / pharmacology*
  • China / epidemiology
  • Communicable Diseases, Emerging / epidemiology*
  • Communicable Diseases, Emerging / microbiology*
  • Female
  • Genes, Bacterial
  • Humans
  • Linezolid / pharmacology*
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Multilocus Sequence Typing
  • Mutation
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology*
  • Staphylococcus epidermidis / drug effects*
  • Staphylococcus epidermidis / genetics
  • Teicoplanin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Teicoplanin
  • Linezolid