Limited antibody specificity compromises epitranscriptomic analyses

Mark Helm; Frank Lyko; Yuri Motorin

doi:10.1038/s41467-019-13684-3

Limited antibody specificity compromises epitranscriptomic analyses

Nat Commun. 2019 Dec 11;10(1):5669. doi: 10.1038/s41467-019-13684-3.

Authors

Mark Helm¹, Frank Lyko², Yuri Motorin³

Affiliations

¹ Institute of Pharmacy and Biochemistry, Johannes Gutenberg-University Mainz, Staudingerweg 5, 55128, Mainz, Germany. [email protected].
² Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany. [email protected].
³ IMoPA, UMR7365 CNRS-University of Lorraine, Biopôle, 9 Avenue de la Forêt de Haye, 54505, Vandœuvre-lès-Nancy, France. [email protected].

Abstract

A controversial discussion on the occurrence of the RNA modification m¹A in mRNA takes a new turn, as an antibody with a central role in modification mapping was shown to also bind mRNA cap structures.

MeSH terms

Animals
Antibodies / genetics
Antibodies / metabolism*
Antibody Specificity
DNA Methylation
Epigenesis, Genetic*
Gene Expression Regulation
Humans
Protein Binding
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transcriptome*

Substances

Antibodies
RNA, Messenger