Activity-based protein profiling in bacteria: Applications for identification of therapeutic targets and characterization of microbial communities

Curr Opin Chem Biol. 2020 Feb:54:45-53. doi: 10.1016/j.cbpa.2019.10.007. Epub 2019 Dec 10.

Abstract

Activity-based protein profiling (ABPP) is a robust chemoproteomic technique that uses activity-based probes to globally measure endogenous enzymatic activity in complex proteomes. It has been utilized extensively to characterize human disease states and identify druggable targets in diverse disease conditions. ABPP has also recently found applications in microbiology. This includes using activity-based probes (ABPs) for functional studies of pathogenic bacteria as well as complex communities within a microbiome. This review will focus on recent advances in the use of ABPs to profile enzyme activity in disease models, screen for selective inhibitors of key enzymes, and develop imaging tools to better understand the host-bacterial interface.

Keywords: Activity-based protein profiling; Bacteria; Microbiome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Bacteria / chemistry
  • Bacteria / enzymology*
  • Bacteria / metabolism
  • Bacterial Infections / microbiology
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Chromatography, Liquid
  • Enzymes / chemistry
  • Enzymes / metabolism
  • Humans
  • Microbiota
  • Protein Array Analysis / methods
  • Proteomics / methods
  • Tandem Mass Spectrometry

Substances

  • Bacterial Proteins
  • Enzymes