Abstract
Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.
MeSH terms
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Acrylic Resins / chemistry
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Acrylic Resins / metabolism
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DNA, Catalytic / metabolism*
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Gels / chemistry
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Gels / metabolism
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Immobilized Nucleic Acids / chemistry
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Immobilized Nucleic Acids / metabolism
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Magnesium / chemistry
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Magnesium / metabolism
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Nucleic Acid Amplification Techniques
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Nucleic Acids / chemistry
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Nucleic Acids / metabolism*
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Particle Size
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Surface Properties
Substances
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Acrylic Resins
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DNA, Catalytic
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Gels
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Immobilized Nucleic Acids
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Nucleic Acids
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polyacrylamide
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Magnesium