Dual-binding benzene and rhodamine B conjugate derivatives as fluorescent chemodosimeter for hypochlorite in living cell imaging

Di Zhang; Zhiwei Ma; Yanliang Wang; Haiyan Yin; Man Li; Yun Wang; Hongqi Wang; Bin Jia; Jihong Liu

doi:10.1016/j.saa.2019.117908

Dual-binding benzene and rhodamine B conjugate derivatives as fluorescent chemodosimeter for hypochlorite in living cell imaging

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 15:229:117908. doi: 10.1016/j.saa.2019.117908. Epub 2019 Dec 6.

Authors

Di Zhang¹, Zhiwei Ma², Yanliang Wang³, Haiyan Yin¹, Man Li¹, Yun Wang¹, Hongqi Wang¹, Bin Jia⁴, Jihong Liu⁵

Affiliations

¹ Henan Cereal Quality and Safety Testing Key Laboratory, Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
² Henan Cereal Quality and Safety Testing Key Laboratory, Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Faculty of Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
³ Department of Nephrology, Henan Provincial Key Laboratory of Kidney Disease and Immunology, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), Zhengzhou, Henan 450003, China.
⁴ Henan Cereal Quality and Safety Testing Key Laboratory, Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Electronic address: [email protected].
⁵ Henan Cereal Quality and Safety Testing Key Laboratory, Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Electronic address: [email protected].

PMID: 31841672
DOI: 10.1016/j.saa.2019.117908

Abstract

A new probe (SRh) which based on dual-binding benzene and rhodamine B conjugate derivatives for hypochlorite detection was developed. By desulfurization effect, probe SRh displayed"Off-On" switching in its fluorogenic and chromogenic responses to hypochlorite. The detection limit of ClO^- was at a low level (up to 2.43 nM). Moreover, probe SRh has been applied in bioimaging with good biocompatibility and low cytotoxicity.

Keywords: Bioimaging; Desulfurization; Fluorescence; Hypochlorite; Rhodamine.

MeSH terms

Benzene / chemistry*
Cell Line
Fluorescent Dyes* / chemical synthesis
Fluorescent Dyes* / chemistry
Fluorescent Dyes* / pharmacology
Humans
Hypochlorous Acid / metabolism*
Optical Imaging*
Rhodamines / chemistry*

Substances

Fluorescent Dyes
Rhodamines
Hypochlorous Acid
Benzene
rhodamine B