Chlorantraniliprole induces adipogenesis in 3T3-L1 adipocytes via the AMPKα pathway but not the ER stress pathway

Food Chem. 2020 May 1:311:125953. doi: 10.1016/j.foodchem.2019.125953. Epub 2019 Dec 3.

Abstract

Flubendiamide, a ryanoid family insecticide, has been linked to elevated adipogenesis. However, the influence on adipogenesis of chlorantraniliprole, another widely-used ryanoid-class insecticide in agriculture, remains unknown. Therefore, the influence of chlorantraniliprole on adipogenesis was studied in 3T3-L1 cells. Chlorantraniliprole enhanced TG content and upregulated the expression of C/EBPα, PPARγ, and ACC, three key adipogenic regulators. Chlorantraniliprole decreased the phosphorylation of AMPK, while it had no influence on the expression of endoplasmic reticulum stress regulators. The influence of chlorantraniliprole on adipogenesis was abolished by AMPKα activation. Collectively, the results indicate that chlorantraniliprole enhances adipogenesis through the AMPKα pathway, but not the ER stress-mediated pathway.

Keywords: Adipogenesis; Chlorantraniliprole; Food chemical pollutant; Ryanoid.

MeSH terms

  • 3T3-L1 Cells
  • AMP-Activated Protein Kinases / genetics
  • AMP-Activated Protein Kinases / metabolism*
  • Adipocytes / drug effects*
  • Adipocytes / metabolism
  • Adipogenesis / drug effects*
  • Animals
  • CCAAT-Enhancer-Binding Protein-alpha / genetics
  • CCAAT-Enhancer-Binding Protein-alpha / metabolism
  • Endoplasmic Reticulum Stress / drug effects*
  • Insecticides / pharmacology*
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Phosphorylation / drug effects
  • Signal Transduction / drug effects
  • ortho-Aminobenzoates / pharmacology*

Substances

  • CCAAT-Enhancer-Binding Protein-alpha
  • Insecticides
  • PPAR gamma
  • ortho-Aminobenzoates
  • chlorantranilipole
  • AMP-Activated Protein Kinases