An amide-based sulfenamide prodrug of gamma secretase inhibitor BMS-708163 delivers parent drug from an oral conventional solid dosage form in male beagle dog

Bioorg Med Chem Lett. 2020 Feb 1;30(3):126856. doi: 10.1016/j.bmcl.2019.126856. Epub 2019 Dec 16.

Abstract

The objective of this Letter is to report the first (to our knowledge) in vivo proof of concept for a sulfenamide prodrug to orally deliver a poorly soluble drug containing a weakly-acidic NH-acid from a conventional solid dosage formulation. This proof of concept was established using BMS-708163 (1), a gamma secretase inhibitor containing a weakly acidic primary amide NH-acid as the chemical handle for attaching a series of thiol-based promoieties via a sulfenamide linkage. Aqueous stabilities and solubilities are reported for a series of six sulfenamide prodrugs (2-7) of 1. The sulfenamide prodrug containing the cysteine methyl ester promoiety (5) was chosen for a orally-dosed PK study in male beagle dog comparing a solubilized formulation of 1 against a solid dosage form of 5 in a cross-over fashion at an equivalent molar dose of 3 mg/kg. Prodrug 5 delivered essentially a superimposable PK profile of 1 compared to the solubilized formulation of 1, without any detectable exposure of 5 in systemic circulation.

Keywords: Drug delivery; NH-acid; Oral bioavailability; Solubilization; Sulfenamide prodrug.

MeSH terms

  • Administration, Oral
  • Amyloid Precursor Protein Secretases / antagonists & inhibitors*
  • Amyloid Precursor Protein Secretases / metabolism
  • Animals
  • Capsules / chemistry
  • Dogs
  • Drug Stability
  • Half-Life
  • Male
  • Oxadiazoles / chemistry*
  • Prodrugs / chemical synthesis
  • Prodrugs / chemistry*
  • Prodrugs / pharmacokinetics
  • Solubility
  • Sulfamerazine / chemical synthesis
  • Sulfamerazine / chemistry*
  • Sulfamerazine / pharmacokinetics
  • Sulfonamides / chemistry*

Substances

  • BMS 708163
  • Capsules
  • Oxadiazoles
  • Prodrugs
  • Sulfonamides
  • sulfenamide
  • Amyloid Precursor Protein Secretases
  • Sulfamerazine