Matrix Metalloproteinases (MMP-2,-3,-9) Gene Polymorphisms in Cases of Benign Vocal Fold Lesions and Laryngeal Carcinoma

In Vivo. 2020 Jan-Feb;34(1):267-274. doi: 10.21873/invivo.11770.

Abstract

Background/aim: The matrix metalloproteinases (MMP) play an important role in the physiological and pathological remodeling of tissues including carcinogenesis. The study's aim was to assess the relations between MMP-2(-735C/T), MMP-2(-1306C/T), MMP-9(-1562C/T), and MMP-3(-11715A/6A) polymorphisms, and clinical/morphological manifestation of laryngeal squamous cell carcinoma (LSCC) and benign vocal fold lesions (BVFL).

Patients and methods: Two hundred and seventeen patients with LSCC and BVFL and 458 controls were included in this study. The genotyping was performed using the real-time polymerase chain reaction method.

Results: The MMP-2(-1306C/T) C/T genotype was significantly rarer among the patients with moderate-poorly differentiated LSCC compared to the control group, however the MMP-3(-11715A/6A) 6A/6A genotype was significantly more frequent compared to controls. Smoking and 6A/6A genotype of MMP-3(-11715A/6A) polymorphism were associated with increased odds of LSCC risk. No associations between MMP genotypes and BVFL were found.

Conclusion: Smoking and MMP-3 (-11715A/6A) 6A/6A genotype may cause a higher risk for developing LSCC.

Keywords: Laryngeal squamous cell carcinoma; benign vocal fold lesions; gene polymorphism; matrix metalloproteinases.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / genetics
  • Case-Control Studies
  • Female
  • Follow-Up Studies
  • Genetic Predisposition to Disease
  • Humans
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / pathology
  • Male
  • Matrix Metalloproteinase 2 / genetics*
  • Matrix Metalloproteinase 3 / genetics*
  • Matrix Metalloproteinase 9 / genetics*
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Prognosis
  • Promoter Regions, Genetic
  • Vocal Cords / metabolism
  • Vocal Cords / pathology*
  • Voice Disorders / genetics*
  • Voice Disorders / pathology
  • Young Adult

Substances

  • Biomarkers, Tumor
  • MMP3 protein, human
  • Matrix Metalloproteinase 3
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9