Novel halochromic cellulose nanowhiskers from rice straw: Visual detection of urea

Tawfik A Khattab; Moustafa M G Fouda; Mohamed Rehan; Mohammad K Okla; Saudi A Alamri; Ibrahim A Alaraidh; Abdullah A Al-Ghamdi; Walid H Soufan; Eslam M Abdelsalam; Ahmed A Allam

doi:10.1016/j.carbpol.2019.115740

Novel halochromic cellulose nanowhiskers from rice straw: Visual detection of urea

Carbohydr Polym. 2020 Mar 1:231:115740. doi: 10.1016/j.carbpol.2019.115740. Epub 2019 Dec 13.

Authors

Affiliations

¹ Dyeing, Printing and Auxiliaries Department, Textile Industries Research Division, National Research Centre, 33 El-Buhouth Street, Dokki, Cairo 12622, Egypt.
² Pretreatment and Finishing of Cellulosic-based Fibers Department, Textile Industries Research Division, National Research Centre, 33 El-Buhouth Street, Dokki, Cairo 12622, Egypt.
³ Pretreatment and Finishing of Cellulosic-based Fibers Department, Textile Industries Research Division, National Research Centre, 33 El-Buhouth Street, Dokki, Cairo 12622, Egypt. Electronic address: [email protected].
⁴ Department of Biology, College of Science, King Saud University, Riyadh,11451 Saudi Arabia.
⁵ Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh,11451 Saudi Arabia.
⁶ Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, 65211, Egypt.

PMID: 31888855
DOI: 10.1016/j.carbpol.2019.115740

Abstract

Colorimetric nanocomposite film sensor was fabricated by incorporating TCFH spectroscopic probe into cellulose nanowhiskers (CNW)/Urease enzyme matrix. CNW-TCFH can be used as disposable molecular biosensor in which CNW is the probe carrier comprising high surface area-to-volume ratio, urease is the catalyst and TCFH is the molecular probe. Tricyanofuran-hydrazone (TCFH) spectroscopic probe was prepared. UV-vis absorption spectra demonstrated solvatochromic behavior and a reversible color change of the tricyanofuran-hydrazone probe solution in acetone under acid/base conditions. CNW were reinforced with sodium alginate biopolymer to introduce biocomposite film. This CNW-TCFH film biosensor responds through visible color shift from light yellow to pink when exposed to urea in aqueous media. The morphology properties of CNW and CNW-TCFH films were examined by different tools. The photophysical properties of the prepared TCFH probe, including solvatochromism and pH sensory, were also studied.

Keywords: Biosensor; Cellulose nanowhiskers; Halochromism; Rice straw; Urea; Urease.