The increased IL-17-producing γδT cells promote tumor cell proliferation and migration in neuroblastoma

Hui Zhang; Wenjia Chai; Wei Yang; Wei Han; Wenjun Mou; Yue Xi; Xi Chen; Hui Wang; Wei Wang; Hong Qin; Huanmin Wang; Xiaoli Ma; Xiaolin Wang; Jingang Gui

doi:10.1016/j.clim.2020.108343

The increased IL-17-producing γδT cells promote tumor cell proliferation and migration in neuroblastoma

Clin Immunol. 2020 Feb:211:108343. doi: 10.1016/j.clim.2020.108343. Epub 2020 Jan 10.

Authors

Hui Zhang¹, Wenjia Chai¹, Wei Yang², Wei Han², Wenjun Mou¹, Yue Xi¹, Xi Chen¹, Hui Wang¹, Wei Wang¹, Hong Qin², Huanmin Wang², Xiaoli Ma³, Xiaolin Wang⁴, Jingang Gui⁵

Affiliations

¹ Laboratory of Tumor Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
² Department of Surgical Oncology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
³ Hematology Oncology Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
⁴ Laboratory of Tumor Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. Electronic address: [email protected].
⁵ Laboratory of Tumor Immunology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. Electronic address: [email protected].

PMID: 31931123
DOI: 10.1016/j.clim.2020.108343

Abstract

Neuroblastoma (NB) is the most common solid extracranial malignancy in children with a considerable chance of metastatic progression. Prevalent evidence supports the anti-tumor role of γδT cells and these cells have been testing in clinical trials for constraining tumor growth. A small subpopulation of γδT cells releasing IL-17, however, were demonstrated to exert tumor-promoting effects in many aspects. In this study, we found an augment of IL-17⁺ γδT cells both in in vitro PAM-stimulated γδT-cell expanding culture and circulating γδT cells in NB patients. These patient-origin cells expanded in vitro by PAM in the presence of IL-17 polarizing condition were shown to promote the proliferation and migration of NB cells. Furthermore, an intrinsic preference for IL-17 polarization in NB γδT cells was revealed by mRNA microarray and Western Blot, which pointed to an up-regulated expression of multiple Th17-development related genes in addition to an increased phosphorylation level of STAT3.

Keywords: IL-17-producing γδT cells; Migration; Neuroblastoma; Proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Movement
Cell Proliferation
Child, Preschool
Female
Humans
Interleukin-17 / immunology*
Intraepithelial Lymphocytes / immunology*
Male
Neuroblastoma / immunology*
Neuroblastoma / pathology

Substances

Interleukin-17