The association between surfactant protein B gene variation and bronchopulmonary dysplasia in Chinese premature newborns

Objective: This study aimed to correlate the pulmonary surfactant B (SP-B) gene variation with bronchopulmonary dysplasia (BPD) in ethnic Han, Chinese, premature newborns.

Method: 47 newborns with BPD and 55 controls without BPD were included. Genomic DNA was extracted from cord or artery blood. Genotyping for the SP-B gene was performed by polymerase chain reaction or gene sequencing, and the clinical characteristics were also analyzed.

Results: Two types of SP-B gene variations in Exon 2 or Exon 5 were discovered, including V1 (Exon 2: c.[5A > C] + [5A > C] or c.[5A > C] + [=]) and V2 (Exon 5: c.[428C > T] + [428C > T] or c.[428C > T] + [=]). In the BPD group, there were 33 newborns with gene variations, of which type V1 and V2 accounted for 18 and 15 respectively. In the control group, there were 19 newborns with gene variations, of which type V1 and V2 accounted for 7 and 12 respectively. There was a significant difference between the two groups in type V1 variation (X²=8.956, P < 0.05), and V1 variation was more likely associated with BPD occurrence. Logistic regression analysis showed that gene variation, premature rupture of membranes, birth weight, and the duration of mechanical ventilation were associated with BPD development. Among them, gene variation and premature rupture of the membranes were risk factors for BPD development.

Conclusions: The exon 2 or 5 of SP-B gene variations were associated with the BPD in Chinese premature newborns, and the type V1: Exon 2: c.[5A > C] + [5A > C] or c.[5A > C] + [=] was a risk factor for the development of BPD.