Analysis of human papillomavirus 16 E6/E7 and L1 in the bronchial brushing cells of patients with squamous cell carcinoma of the lungs

A type of high-risk human papillomavirus (HPV), HPV16 takes part in lung carcinogenesis. E6 and E7 are the major oncoproteins of high-risk HPV, and L1 is the major capsid protein. In this study, we detected their mRNA expressions and analyzed their relationship in the bronchial brushing cells of 211 patients with malignant lesions (squamous cell carcinoma of the lungs) and benign lesions (pneumonia and tuberculosis) by quantitative real-time PCR. HPV16 E6, E7, and L1 mRNA expressions in the malignant group were statistically higher than in benign group (P<0.05), and their mRNA expressions in the squamous cell carcinoma of the lung group were statistically higher than in pneumonia group (P<0.05). There was a negative correlation between L1 and E6 expression in the squamous cell carcinoma of the lungs group (Spearman correlation coefficient r=-0.498, P=0.000). An ROC curve shows that the combination of L1 and E6 is a significant predictor for the diagnosis of squamous cell carcinoma of the lungs (AUC: 0.878; Sensitivity: 96.00%; Specificity: 77.91%), which could make up for the deficiency of cytologic testing. The combined detection of HPV16 E6 and L1 mRNA expressions in bronchial brushing cells by quantitative real-time PCR has a great significance for the diagnosis of squamous cell carcinoma of the lungs, providing new therapeutic targets for the clinical treatment of squamous cell carcinoma of the lungs.