Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time

Brandon J Davis; Mitchell R O'Connell

doi:10.1016/j.molcel.2019.12.018

Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time

Mol Cell. 2020 Jan 16;77(2):207-209. doi: 10.1016/j.molcel.2019.12.018.

Authors

Brandon J Davis¹, Mitchell R O'Connell²

Affiliations

¹ Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA.
² Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA. Electronic address: [email protected].

PMID: 31951545
DOI: 10.1016/j.molcel.2019.12.018

Abstract

Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 approach to image colocalized or repeat-containing RNAs in real time, as well as demonstrate simultaneous RNA-DNA labeling by using Cas13 and Cas9 in tandem.

Publication types

Comment

MeSH terms

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA
Eyeglasses
RNA*

Substances

RNA
DNA

Grants and funding

R35 GM133462/GM/NIGMS NIH HHS/United States