Reference Human Rotavirus A Genome Sequence from a Previously Vaccinated Child with Diarrhea in Nigeria

T O C Faleye; U E George; C Simsek; O A Arowolo; O M Adewumi; J Matthijnssens; J A Adeniji

doi:10.1128/MRA.01352-19

Reference Human Rotavirus A Genome Sequence from a Previously Vaccinated Child with Diarrhea in Nigeria

Microbiol Resour Announc. 2020 Jan 30;9(5):e01352-19. doi: 10.1128/MRA.01352-19.

Authors

T O C Faleye^{1

2}, U E George³, C Simsek⁴, O A Arowolo⁵, O M Adewumi^{6

7}, J Matthijnssens⁴, J A Adeniji^{2

7

8}

Affiliations

¹ Centre for Human Virology and Genomics, Department of Microbiology, Nigerian Institute of Medical Research, Lagos, Nigeria.
² Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
³ Department of Biological Sciences, Redeemer's University, Ede, Nigeria.
⁴ KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Viral Metagenomics, Leuven, Belgium.
⁵ Department of Veterinary Microbiology, National Veterinary Research Institute, Vom, Nigeria.
⁶ Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria [email protected].
⁷ Infectious Disease Institute, University of Ibadan, Ibadan, Nigeria.
⁸ WHO National Polio Laboratory, University of Ibadan, Ibadan, Nigeria.

Abstract

In 2018, a 26-month-old girl, fully vaccinated with Rotarix in 2016, presented with fever, diarrhea, and vomiting. A rapid test showed that her feces contained rotavirus A (RVA). VP7 reverse transcription-PCR (RT-PCR) and Illumina sequencing showed that a G1P[8] strain with a Wa-like genotype constellation was the etiologic agent. This is the first near-complete RVA genome sequence from Nigeria.