Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species

Methods Mol Biol. 2020:2113:23-29. doi: 10.1007/978-1-0716-0278-2_3.

Abstract

Colocalization single-molecule spectroscopy (CoSMoS) allows studying RNA-protein complexes in the full complexity of their cellular environment at single-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe an approach to monitor multiple RNA species simultaneously to enable direct comparison. This approach represents a technological development to avoid conventional inter-experiment comparisons.

Keywords: CoSMoS; Colocalization single-molecule spectroscopy; RNA spectroscopy; RNA-protein complex; RNP; Single-molecule fluorescence microscopy; Single-molecule spectroscopy; TIRF microscopy.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Extracts / chemistry
  • Fluorescent Dyes / chemistry
  • Microscopy, Fluorescence
  • RNA / chemistry*
  • RNA / metabolism
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Single Molecule Imaging / methods*
  • Staining and Labeling

Substances

  • Cell Extracts
  • Fluorescent Dyes
  • RNA-Binding Proteins
  • RNA