Chemical constituents from shells of Xanthoceras sorbifolium

Gang Chen; Yumeng Xie; Di Zhou; Yanqiu Yang; Jingyu Liu; Yue Hou; Maosheng Cheng; Yang Liu; Ning Li

doi:10.1016/j.phytochem.2020.112288

Chemical constituents from shells of Xanthoceras sorbifolium

Phytochemistry. 2020 Apr:172:112288. doi: 10.1016/j.phytochem.2020.112288. Epub 2020 Feb 8.

Authors

Gang Chen¹, Yumeng Xie¹, Di Zhou¹, Yanqiu Yang², Jingyu Liu², Yue Hou², Maosheng Cheng³, Yang Liu⁴, Ning Li⁵

Affiliations

¹ School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, China.
² School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, China.
³ Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, 110016, China.
⁴ Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, 110016, China. Electronic address: [email protected].
⁵ School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, China. Electronic address: [email protected].

PMID: 32045741
DOI: 10.1016/j.phytochem.2020.112288

Abstract

Three undescribed triterpenes and four previously unreported saponins, along with two known ones, were isolated from shells of Xanthoceras sorbifolium (Sapindaceae). Their structures were elucidated by the interpretation of 1D and 2D NMR data. The nitric oxide (NO) assay revealed that 28-O-isobutyryl-21-O-angeloyl-R₁-barrigenol and 3-O-β-D-6-O-methylglucuronopyranosyl-21,22-di-O-angeloyl-R₁-barrigenol possessed stronger inhibitory effects on LPS-induced NO overproduction (IC₅₀ = 18.5 ± 1.2 and 28.2 ± 1.8 μM, respectively) than the positive drug minocycline (IC₅₀ = 30.1 ± 1.3 μM) in activated BV2 cells. Western blot, RT-qPCR, and docking experiments further validated that the regulation of iNOS and IL-1β expressions was involved in the anti-neuroinflammatory effects of these two compounds.

Keywords: Docking; Microglia; Neuroinflammation; Sapindaceae; Triterpene saponins; Xanthoceras sorbifolium.

MeSH terms

Magnetic Resonance Spectroscopy
Sapindaceae*
Saponins*
Triterpenes*

Substances

Saponins
Triterpenes