Picosecond transient absorption study of photodissociated carboxy hemoglobin and myoglobin

Biophys J. 1988 Sep;54(3):545-9. doi: 10.1016/S0006-3495(88)82987-4.

Abstract

The optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carboxyhemoglobin / metabolism*
  • Carboxyhemoglobin / radiation effects
  • Humans
  • Kinetics
  • Myoglobin / metabolism*
  • Myoglobin / radiation effects
  • Photolysis
  • Spectrophotometry, Ultraviolet
  • Whales

Substances

  • Myoglobin
  • carboxymyoglobin
  • Carboxyhemoglobin