The prevention of autolysis in stored corneas by lysosome stabilization. A histochemical study

Can J Ophthalmol. 1977 Jan;12(1):48-53.

Abstract

We wished to determine if dexamethasome, acting as a lysosome stabilizer, could reduce the release and activation of the lysosomal acid hydrolases, and thus retard autolysis of stored corneas. One cornea (experimental) of a rabbit was soaked in a 2 per cent steroid solution and the other cornea (control) in physiological saline for 3 hours at 23 degrees C. The experimental and control corneas were then processed histochemically to show the localization of the lysosomal marker enzymes beta-glucuronidase and acid phosphatase. Compared to the controls the steroid treated corneas showed reduced enzyme activity suggesting that autolysis during storage had been retarded.

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Autolysis / prevention & control*
  • Cornea / enzymology
  • Corneal Transplantation*
  • Dexamethasone / pharmacology*
  • Endothelium / enzymology
  • Epithelium / enzymology
  • Glucuronidase / metabolism
  • Histocytochemistry
  • Lysosomes / enzymology*
  • Organ Preservation / methods*
  • Rabbits
  • Tissue Preservation / methods*

Substances

  • Dexamethasone
  • Acid Phosphatase
  • Glucuronidase